A simple, highly sensitive, and selective fluorescent method was developed to detect adrenaline, also known as epinephrine (EP). Adrenaline is a crucial hormone, neurotransmitter, and a substance used in drugs and doping. It utilized Fe3O4-APTS (3-aminopropyltriethoxysilane) nanoparticles to enhance the fluorescence of MNPs. Characterization of the synthesized MNPs was conducted through transmission electron microscopy (TEM), field emission scanning electron microscopy (FESEM), X-ray diffraction (XRD) and Fourier transform infrared spectroscopy (FTIR). Fe3O4 -APTS demonstrated the highest extraction rates of adrenaline from aqueous solutions, synthetic urine, and human urine, with extraction ratios of 99%,96%, and 87% respectively. The detection limit (LOD) and the quantitative limit (LOQ) of adrenaline were determined using fluorescence spectroscopy at room temperature and were found to be 0.038871 μg/L and 0.11779 μg/L. A linear relationship between fluorescence intensity and adrenaline concentrations was established in the range of 4 to 12 μg mL−1. In addition, the experiment achieved approximately 90% disintegration of the bond between Fe3O4-APTS and adrenaline in an aqueous solution. It also achieved 84% disintegration of adrenaline in human urine. Furthermore, the successful application of this method to synthetic and human urine samples highlights its potential for diagnostic purposes
Khinteel,M. J and Alfarhani,B. F (2024). Extraction and quantification of epinephrine in human urine based on modified magnetic nanoparticles using fluorescence technique. Chemical Review and Letters, 7(5), 860-872. doi: 10.22034/crl.2024.471918.1394
MLA
Khinteel,M. J, and Alfarhani,B. F. "Extraction and quantification of epinephrine in human urine based on modified magnetic nanoparticles using fluorescence technique", Chemical Review and Letters, 7, 5, 2024, 860-872. doi: 10.22034/crl.2024.471918.1394
HARVARD
Khinteel M. J, Alfarhani B. F (2024). 'Extraction and quantification of epinephrine in human urine based on modified magnetic nanoparticles using fluorescence technique', Chemical Review and Letters, 7(5), pp. 860-872. doi: 10.22034/crl.2024.471918.1394
CHICAGO
M. J Khinteel and B. F Alfarhani, "Extraction and quantification of epinephrine in human urine based on modified magnetic nanoparticles using fluorescence technique," Chemical Review and Letters, 7 5 (2024): 860-872, doi: 10.22034/crl.2024.471918.1394
VANCOUVER
Khinteel M. J, Alfarhani B. F Extraction and quantification of epinephrine in human urine based on modified magnetic nanoparticles using fluorescence technique. Chem. Rev. Lett., 2024; 7(5): 860-872. doi: 10.22034/crl.2024.471918.1394
